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. 2018 Jan 19;8:1264. doi: 10.1038/s41598-018-19704-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dexamethasone mediated miR-155 up-regulation does not affect adipogenesis. Murine 3T3-L1 preadipocytes were transfected with miR-155 inhibitor or negative control. Cells were induced to differentiate in the presence of 500 µM IBMX (M), 100 nM insulin (I), and supplemented with either solvent control (MI) or 250 nM dexamethasone (MID) as indicated. (a) After 24 hours, miRNA was isolated, and the expression levels of miR-155 were quantified by real-time qPCR. Levels were normalized to endogenous RNU6 and expressed as fold over MI. Results from 3 separate experiments are graphically represented as mean ± S.E.M. (b) At day 6 of differentiation, RNA was isolated, and the mRNA levels of and adipogenic markers were quantified by real-time qPCR. Levels were normalized to endogenous β-actin levels and expressed as fold over MID + negative control. Results from 3 separate experiments are graphically represented as mean ± S.E.M. **Denotes p < 0.01 for the indicated comparisons, as assessed by one-way ANOVA with Tukey’s post-hoc tests.