Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 19;8:1204. doi: 10.1038/s41598-018-19579-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

MarvelD3 depletion targeting a neural crest progenitor inhibits twist expression. (A) Scheme of the lateral view of a 32-cell stage embryo (adapted from Xenbase). The blastomeres are identified with from dorsal (D) to ventral (V) as A1 (mainly neural plate), A3 (mainly neural crest and epidermis) and A4 (mainly epidermis); An, animal; Vg, vegetal. (B) Neural crest induction (stage 15) was quantified by analysing twist expression by WISH in embryos injected at 32-cell stage either in the A1, A3 or A4 blastomere; Student t-test p values and the number of embryos analysed is indicated on the graph; blue bar, normal neural crest; red bar, reduced neural crest. Expression of the neural crest marker, twist by WISH in embryos injected in A1 (C), A3 (D) or A4 (E) blastomere, respectively at 32-cell stage. β-galactosidase (light blue staining) is expressed in the cells derived from the targeted blastomere. Note the reduction of twist expression only in A3 injected embryos. Scale bar, 500 μm.