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. 2018 Jan 19;8:1188. doi: 10.1038/s41598-018-19391-1

Figure 2.

Figure 2

Efficiencies of IMNs to capture target cells. (A) The capture efficiencies at different concentrations of anti-EpCAM-MNs to capture MCF7 cells (black) and anti-FRα-MNs to capture A2780 cells (gray). (B) Specificity detection of IMNs. Black column shows capture efficiencies of anti-EpCAM-MNs to MCF7 and Jurkat cells and MNs to MCF7 cells. The gray column shows the capture efficiencies of anti-FRα-MNs to A2780 and A549 cells and MNs to A2780 cells. (C) The capture efficiencies at different incubation times of anti-EpCAM-MNs to capture MCF7 cells (black) and anti-FRα-MNs to capture A2780 cells (gray). (D) The capture efficiencies of anti-EpCAM-MNs (light gray) or anti-FRα-MNs (dark gray) used alone or in combination (white) to capture MCF7, A2780, and five types of NSCLC cells; the black column shows the capture efficiencies of unmodified MNs to cells. (E) Capture efficiencies in mimicking clinical samples of anti-EpCAM-MNs (light gray), anti-FRα-MNs (dark gray), combined anti-EpCAM-MNs and anti-FRα-MNs (white), and unmodified MNs (black) to capture SPC-A-1, H157, or a mixture of A2780 and MCF7 cells. (F) The regression analyses plots of recovered vs the number of spiked MCF7 cells detected by anti-EpCAM-MNs. (G) The regression analyses plots of recovered vs the number of spiked A2780 cells detected by anti- FRα-MNs. (F) The regression analyses plots of recovered vs the number of spiked SPC-A-1 cells detected by the combination of anti-EpCAM-MNs and anti-FRα-MNs.