Figure 7.
H3K79 methylation is involved in the IFN response to influenza virus. MDCK, MDCK V2 or MDCK Npro cells were plated alone or with Dot1L inhibitor (1 μM, 48 h), then infected (m.o.i. 10−3) with PR8hv (A) or CAL 07 (B). Virus titer was determined by plaque assay on MDCK cells at various hpi. In all cases, inhibitor was present throughout the experiment in treated cells. (C) MDCK, MDCK V2 or MDCK Npro cells were plated alone or with Dot1L inhibitor (1 μM, 48 h), then PR8hv-infected (1 PFU/cell). At indicated hpi, RNA was extracted and used for qPCR detection of IFNβ, ISG56 and MX1 genes. Student’s t test with Welch’s correction; 3 technical replicates of 3 independent experiments were performed.