Figure 6.

Involvement of BAFF in MΦ-mediated protection of ARP-1 cells. Western blot analyses showed that (a) ARP-1 cells cultured alone or co-cultured in direct contact with MΦs were detected for activation and cleavage of PARP and caspase-3, either PBS-treated or bort-treated (5 nM, 24 h). (b) ARP-1 cells cultured alone or co-cultured in direct contact with MΦs in the presence of BAFF-neutralizing antibody or control IgG2B and ARP-1 cells cultured alone or co-cultured in direct contact with sictl-MΦs or siBAFF-MΦs were detected for activation and cleavage of PARP and caspase-3, either PBS-treated or bort-treated (5 nM, 24 h). (c) Expression of pAkt (Ser473), Akt, pErk1/2 (T202/Y204), Erk1/2, pSrc (Y416), Src, and GAPDH in ARP-1 cells cultured alone or directly co-cultured with MΦs in the presence of BAFF-neutralizing antibody or control IgG2B and in ARP-1 cells cultured alone or directly co-cultured with sictl-MΦs or siBAFF-MΦs, either PBS-treated or bort-treated (5 nM, 24 h). (d) Expression of TRAF3, NIK, IKKα, IκBα, and pIκBα (Ser32) in ARP-1 cells directly co-cultured with sictl-MΦs or siBAFF-MΦs, either PBS-treated or bort-treated (5 nM, 24 h). (e) Processing of p100 to p52 and translocation of p52 or p65 to the nucleus in ARP-1 cells directly co-cultured with sictl-MΦs or siBAFF-MΦs, bort-treated (5 nM, 24 h)