Table 1. Number of animals used for all experimental groups.
| Group/time of killing | 1 DPE | 3 DPE | 7 DPE | 10 DPE | 14 DPE | 49 DPE |
|---|---|---|---|---|---|---|
| Intact-epoB | 3 (Array) | |||||
| SCI-vehicle (days post vehicle treatment) | 3 (Array) | 3 (Array) 6 (Immunofluorescence staining) contain ELISA (n=3) 6 (FC) contain ELISA (n=3) 3 (M/M labeling in vivo) | 3 (Array) 6 (immunofluorescence staining) contain ELISA (n=3) 6 (FC) contain ELISA (n=3) | 6 (MPO assay) 6 (immunofluorescence staining) | 3 (Array) 3 (ELISA) | |
| SCI-epoB | 3 (Array) | 6 (Array) 6 (immunofluorescence staining) contain ELISA (n=3) 6 (FC) contain ELISA (n=3) 3 (M/M labeling in vivo) | 3 (Array) 6 (immunofluorescence staining) contain ELISA (n=3) 6 (FC) contain ELISA (n=3) | 6 (MPO assay) 6 (immunofluorescence staining) | 3 (Array) 3 (ELISA) | 9 (BBB) |
| SCI-epoB-M-CSF Ab | 6 (immunofluorescence staining) 6 (FC) | 6 (FC) | 9 (BBB) | |||
| SCI-epoB-MCP-1 Ab | 6 (immunofluorescence staining) | |||||
| SCI-epoB-NAM | 6 (FC) | 6 (FC) | 9 (BBB) | |||
| SCI-epoB -LIX Ab | 6 (MPO assay) 6 (immunofluorescence staining) | 9 (BBB) | ||||
| Total number=198 | 6 | 66 | 42 | 36 | 12 | 36 |
Abbreviations: Ab, antibody; BBB, Basso, Beattie, and Bresnahan; DPE, day post epoB treatment; ELISA, enzyme-linked immunosorbent assay; epoB, epothilone B; FC, flow cytometry; LIX, C–X–C motif chemokine 5; MCP-1, monocyte chemoattractant protein-1; M-CSF, macrophage colony-stimulating factor; M/M, microglia/macrophages/monocyte; MPO, myeloperoxidase; NAM, neutralizing antibody mix; SCI, spinal cord injury