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. 2018 Jan 19;8:1233. doi: 10.1038/s41598-017-18823-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative RT-PCR based validation of expression of mature miRNAs in imbibed seeds, in comparison to dry seeds, in Arabidopsis thaliana. (The expression profiles obtained from qRT-PCR slightly varied with the microarray based miRNA expressions, probably due to the pulling of total RNAs during microarray). The qRT-PCR validation of miRNAs were done at six different germination stages as 12 h/RT, 12 h/4 °C, 24 h/RT, 24 h/4 °C, 48 h/RT and 48 h/4 °C each compared to dry seed. The expression values presented here were the means of three biological replicates ± standard deviation (SD). The Arabidopsis ACTIN7 was used for each samples as an endogenous control. (a) ath-miR165/166; (b) ath-miR172a; (c) ath-miR390b; (d) ath-miR160a; (e) ath-miR156h; (f) ath-miR157a/or, c/or, d. In Arabidopsis, miR157a, c and d have the same mature sequence. Asterisks indicate significant statistical differences: ***P < 0.001, **P < 0.01, *P < 0.05 (One-way ANOVA).