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. 2018 Jan 19;8:1233. doi: 10.1038/s41598-017-18823-8

Figure 4.

Figure 4

Quantitative RT-PCR based validation of different target genes in imbibed seeds in comparison to dry seeds in Arabidopsis thaliana. The qRT-PCR validation of miRNAs and the targets were done at six different germination stages as 12 h/RT, 12 h/4 °C, 24 h/RT, 24 h/4 °C, 48 h/RT and 48 h/4 °C each compared to dry seed. The expression values presented here were the means of three biological replicates ± SD. The Arabidopsis ACTIN7 was used for each sample as an endogenous control.(a–d) targets of miR165/166; (a) PHB; (b) PHV; (c) ATHB8; (d) ATHB15. (e–g) targets of miR160; (e) ARF10; (f) ARF16; (g) ARF17. (h,i) targets of miR156/or, miR157; (h) SPL3; (i) SPL9. Asterisks indicate significant statistical differences, ***P < 0.001, **P < 0.01, *P < 0.05 (One-way ANOVA).