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. 2018 Jan 19;8:1233. doi: 10.1038/s41598-017-18823-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative RT-PCR based validation of various predicted target genes in imbibed vs dry seeds in Arabidopsis. The target genes were selected through psRNA Target tool based on their expectation values. The qRT-PCR validation of the targets were done at six different germination stages as 12 h/RT, 12 h/4 °C, 24 h/RT, 24 h/4 °C, 48 h/RT and 48 h/4 °C each compared to dry seed. The expression values presented here were the means of three biological replicates ± SD. The Arabidopsis ACTIN7 was used for each sample as an endogenous control. Expression of (a) NF-YA8-target of miR169; (b) PPR superfamily- target of miR161.1; (c) PHO2- target of miR399; (d) AGL16- target of miR824; (e) CIP4.1 or CIP4- target of miR834; (f) R3H- target of miR854. Asterisks indicate significant statistical differences; ***P < 0.001, **P < 0.01, *P < 0.05 (One-way ANOVA).