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. 2018 Jan 19;8:1233. doi: 10.1038/s41598-017-18823-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial expression of pMIR390b::GUS in germinating seeds, function of miR390, and expression of ARF2, ARF3, and ARF4 during seed germination. (a) GUS expression of pMIR390b::GUS at 24 h/RT imbibed condition; (b) GUS expression of pMIR390b::GUS at 24 h/4 °C imbibed condition; (c) Negative control of GUS assay at 24 h/4 °C imbibed Col-0 seed. 24 h/RT-imbibed seeds were showing the higher expression of miR390b compare to 24 h/4 °C-imbibed seeds, which is similar to the expression pattern of the qRT-PCR based validation result of miR390b; (d)The model depicting the role of miR390 inthe biogenesis of tasiR-ARFs and regulation of ARF2/3/4 (According to Marin et al., 2010). (e–g) qRT-PCR based expression pattern of ARF2/3/4, which are the targets of tasiR-ARF; (e) transcript level of target ARF2; (f) transcript level of targetARF3; (g) transcript level of target ARF4.