Conditional Deletion of Nanog Reduces PGC Numbers
(A) Strategy for Nanog conditional knockout. Nanogflox/flox females are crossed with Nanog+/−; Prdm1-Cre male mice. As Prdm1-Cre is heterozygous, one in four embryos will have germline deletion of Nanog (NanogΔ/−).
(B) E11.5 genital ridge sections from NanogΔ/− and control embryos immunostained for Nanog, Dazl, and GFP and counterstained with 4',6-diamidino-2-phenylindole (DAPI) (scale bar, 50 μm).
(C) Cell counts of PGCs in NanogΔ/− and control genital ridges at E11.5. PGCs identified by co-staining for Oct4 and either Dazl or Mvh. The mean (± SD) of two biological and technical replicates for each sample are shown. ∗p < 0.05 (unpaired Student’s t test).
(D) E12.5 genital ridges from NanogΔ/− and control embryos immunostained for GFP and Mvh and counterstained with DAPI (scale bar, 50 μm).
(E) Table of breeding data for adult NanogΔ/− mice. Both male (row 2) and female (row 4 and 5) NanogΔ/− mice are fertile.
See also Figure S1.