Figure 2.

Contribution of Nanog-Null ESCs to Adult Chimeras, Including the Germline

(A) Strategy for generation Nanog^Δ/− (Nanog-null) clonal ESC lines.

(B) Phase contrast and fluorescence images of parental and Nanog-null ESC lines (scale bar, 100 μm).

(C) Oct4, Nanog, and GFP immunostaining of parental and Nanog-null ESC lines (scale bar, 100 μm).

(D) Chimeras generated from Nanog-null ESCs, C57BL/6 mates, and agouti and black pups. High-contribution chimeras generated by injection of agouti Nanog-null ESCs into C57BL/6 blastocysts.

(E) Summary of blastocyst injections and germline contribution of four clonal Nanog-null ESC lines.

See also Figures S2 and S3.