Table 1.
RyR Parameter Properties Estimated from DNA-PAINT Data
| RyR cluster sizea,b | 8.81 ± 3.56 (n = 17 cells, 10 animals) |
| Nearest neighbor distancea,c | 40.1 ± 0.9 nm (n = 1,802 clusters, 8 animals) |
| Average of distances to 4 nearest neighborsa,c | 58.9 ± 0.9 nm (n = 1,802 clusters, 8 animals) |
| RyR packing density within clustera,d | 3,300 nm–2 (n = 391 clusters) |
| JPH2/RyR ratioe,f | 1.38 ± 0.5 (n = 250 clusters, 2 animals) |
a
All measurements shown as mean ± SEM.
b
Estimated from counting punctate densities of all RyR-labeled regions in TIRF DNA-PAINT images.
c
From clusters containing ≥5 RyR.
d
From the slope of the linear slope in scatterplot of cluster area versus qPAINT estimated RyR counts.
e
Shown as mean ± SD of histogram.
f
From qPAINT analysis of RyR and JPH2 exchange-PAINT data.