Figure 2.

Schematic representation of the preparation process for glycomics analysis in mass spectrometry biomarkers research. Protocols for glycoprotein analysis depend on the amount of sample. Since biomarkers research often use tissue or serum/plasma samples, the strategy adopted does not require presence of SDS for protein denaturation but is only applicable if more than 50 mg of protein is available. If you are not interested studding O-glycosylation in the moment, you can storage the peptides/O-glycopeptides and proceed directly to permethylation of N-glycans.