Fig. 1.

Total RNA from yeast in stationary and mid log growth phase treated or untreated with Terminator. a SYBR-Gold stained gel showing RNA treated with Terminator along with untreated RNA. Significant portions of 25S and 18S rRNA are protected from digestion. All conditions started out with the same amount of RNA. b Northern blot, using probes specific for either 5′ or 3′ ends of 25S and 18S ribosomal subunits confirming them to be the large and small subunits of rRNA protected from Terminator digestion. c SYBR-Gold stained gel displaying RNA isolated at mid log and stationary phases and treated with or without Terminator. d Northern Blot using 25S, 18S and 5S probes. Ribosomal 5S was used as loading control