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. 2018 Jan 19;9:6. doi: 10.1186/s13293-018-0164-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effect of short-term diabetes on PGC-1α gene expression, respiratory chain complex IV expression and mitochondrial DNA. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of PGC1-α. b Immunoblot of mitochondrial OXPHOS Complex IV from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graph, with the respective loading control (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. c Total DNA was isolated from non-diabetic Ctrl and STZ rats and run for quantitative real-time PCR to obtain a relative ratio of mt-CoxII (a gene coded on the mitochondrial genome) over 36B4 (a gene coded on nuclear genome), as indicator for relative mt-DNA copy number. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sexby diabetes were analyzed using two-way ANOVA (significance: ##p < 0.01; ###p < 0.001) followed by Bonferroni post hoc test (significance: *p < 0.05; **p < 0.01)