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Differential expression of siglec-E on splenic myeloid cell populations. Wild-type (WT) and siglec-E-deficient splenocytes were gated for dendritic cells (DCs) (CD11c, MHCII double-positive cells), macrophages (F480 positive cells), and granulocytes (Gr-1-positive cells) and analyzed for siglec-E expression by flow cytometry. Two independent biological replicates were performed for each genotype.
