Figure 4.

Other inhibitory CD33-related siglecs do not compensate for siglec-E deficiency in bone marrow-derived macrophages (BMDM). Relative gene expression of siglec-E, siglec-F, and siglec-G in BMDM untreated or stimulated with 10 ng/ml lipopolysaccharide (LPS) for 48 h from wild-type and siglec-E-deficient mice. Gene expression is shown relative to GAPDH, using untreated WT BMDM as calibrator. WT bone marrow cells were used as a control for siglecs-E, -F, and -G as they are expressed in developing neutrophils, eosinophils, and B cells, respectively, and show the expected gene expression profiles.