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. 2018 Jan 15;8:1926. doi: 10.3389/fimmu.2017.01926

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Copyright © 2018 Nagala, McKenzie, Richards, Sharma, Thomson, Mastroeni and Crocker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative proteomics analysis of wild-type (WT) and KO2 bone marrow-derived macrophages (BMDM) activated by lipopolysaccharide (LPS). (A) Volcano plot showing proteomics data derived from four independent biological replicates. WT and KO2 BMDM cells were primed for 3 days with 1 ng/ml LPS and challenged with 100 ng/ml LPS + GolgiStop for 7 h and lysates subjected to quantitative proteomics. The inflammatory mediators are annotated in pink. Gapdh and histone variants are in green and cytokine receptors in blue. (B) GO analysis of proteins that are differentially regulated between WT and KO2 BMDM.