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. Author manuscript; available in PMC: 2018 Jan 20.
Published in final edited form as: J Cell Physiol. 2013 Jan;228(1):225–234. doi: 10.1002/jcp.24126

Figure 6. Inhibition of density-dependent promotion of α-SMA expression by TGF-β neutralizing antibody.

Figure 6

P15 cells were seeded at 500, 5,000 and 50,000 cells/cm2 for 1 week, and immunostained for α-SMA on day 1 (upper panel) and day 7 (lower panel). On day 1, fewer than 0.33% of cells were α-SMA-positive at 500 cells/cm2, but increased to 1.26% at 5000 cells/cm2, and 1.70% at 50,000 cells/cm2. By day 7, such α-SMA expression was increased to 0.4, 1.77, and 4.7 %, respectively (p <0.01* only for 50,000 cells/cm2). At 500 cells/cm2, addition of 5 ng/ml TGF-β for 48 h significantly promoted α-SMA expression, associated with prominent stress fibers (p <0.01*) Addition of 10 μg/ml of anti-TGF-β neutralizing antibody for 48 h significantly inhibited α-SMA expression at 50,000 cells/cm2 (p <0.01*). Bar represents 100 μm.