Fig. 1.

Identification and characterization of amyloid-forming proteins. Spent medium (TDM-glucose) from a P1-deficient S. mutans mutant was concentrated and fractionated by ion-exchange chromatography. (a) ThT fluorescence assay showing ThT uptake by unstirred and stirred (aggregated) samples of pooled fractions corresponding to ion exchange peaks, as well as load and flow-through (FT) fractions. (b) SDS-PAGE of unstirred samples shown in (a), as well as the starting material (load), FT fractions and starting material prior to filtration. (c) SDS-PAGE of purified recombinant protein candidates. (d) ThT fluorescence assay of unstirred and stirred recombinant proteins. ThT uptake was measured at pH 4 and pH 8. Error bars represent the mean±sem of four independent replicates.