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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Anal Bioanal Chem. 2017 Aug 10;410(3):933–941. doi: 10.1007/s00216-017-0548-7

Figure 2.

Figure 2

Pressure-driven injection μCE for off-chip AF 488-labeled (a) Fer (200 nM, N = 21,000 plates/m), (b) CRF (750 nM, N = 77,000 plates/m) and (c) Fer (200 nM, N = 11,000 plates/m)/CRF (750 nM, N = 270,000 plates/m) mixture using a 3-layer PDMS-COC device with 18 ± 1 μm (n=16) channel depth, 24 mm separation channel length, and 200 μm valve spacing. Additional experimental conditions: 20 Hz pump actuation rate, 30 s injection time, 10 mm detection distance, 1500 V μCE voltage, and 25 mM BCB pH 9.9 with 0.05% MC separation buffer.