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Off-chip AF 488-labeled PTB biomarker analysis in an integrated microchip for (a) Fer (50 nM, 30 s cumulative injection time, N = 5600 plates/m), (b) CRF (750 nM, 40 s cumulative injection time, N = 180,000 plates/m) and (c) Fer (100 nM, N = 8800 plates/m)/CRF (750 nM, N = 220,000 plates/m) mixture (30 s cumulative injection time). All parameters except μCE voltage [1500 V for (b)] were the same as in Figure 3b.
