Skip to main content
. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2017 Jul 20;177:140–148. doi: 10.1016/j.jsbmb.2017.07.009

Figure 2. p-STAT1 reduction correlates with IFN-γ inhibition and is independent of total STAT1 protein levels.

Figure 2

A. Western blot analysis was performed on TL-1 protein lysates harvested at time points following calcitriol or vehicle treatment. Representative western blots are shown, with β actin used as a loading control. B. p-STAT1 (Y701) or C. STAT1 results were normalized to total STAT1 or β actin, respectively, and then further normalized to the vehicle control to illustrate changes in protein levels as a result of calcitriol treatment (n=3–7, +/− SEM). D. TL-1 cells were pretreated with calcitriol or ethanol for 1 h and then treated with cycloheximide (10 μg/mL) to inhibit protein synthesis. Lysates were prepared and western blot analysis was performed at the indicated time points for the designated proteins. E. Quantification of D, normalizing STAT1 to β actin. F. TL-1 cells were treated with calcitriol for 4 or 24 h and STAT1 transcript levels were assayed by qPCR. Primary T-LGLL patient PBMCs were treated with calcitriol for 24 h. Results were normalized to the housekeeping gene UBC and then to the ethanol control (for TL-1, n=3, +/− SEM).