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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2017 Jul 20;177:140–148. doi: 10.1016/j.jsbmb.2017.07.009

Figure 3. VDR protein levels increase following calcitriol treatment.

Figure 3

A. Western blot analysis was performed on TL-1 protein lysates harvested at time points following treatment with calcitriol or vehicle control and probed for the VDR. Representative western blots are shown. B. Quantification of western blots from A normalized to β actin (n=3–7, +/− SEM). C. TL-1 cells were pretreated with calcitriol or ethanol for 1 h and then treated with cycloheximide (10 μg/mL) to inhibit protein synthesis. Lysates were prepared at the indicated time points and western blot analysis was performed. D. Quantification of C, normalizing VDR to β actin. E. TL-1 cells were treated with calcitriol for 4 or 24 h and VDR transcript levels were assayed by qPCR. Primary T-LGLL patient PBMCs were treated with calcitriol for 24 h. Results were normalized to the housekeeping gene UBC and then to the ethanol control (for TL-1, n=3, +/− SEM).