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. 2017 Nov 21;72(1):32–42. doi: 10.1007/s11418-017-1144-z

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Fig. 4 — Indirect competitive ELISA to detect antigen. (i) Attach antigen to solid phase. (ii) Incubate free target antigen with primary antibody. (iii) Wash unbound free target antigen and primary antibody out. (iv) Incubate with enzyme-labeled secondary antibody. (v) Develop color with substrate