Fig. 3.

Interaction of the IP₃R1 and σ1R receptor was verified by proximity ligation assay and immunoprecipitation of these receptors. Experiments were performed on NG-108 cells differentiated by dbcAMP. The mutual interaction of the IP₃R1 and σ1R was verified by a proximity ligation assay (a), where red dots show co-localization of these two receptors. Bar represents 20 µm. NCI negative control without IP₃R1 primary antibody, NCS negative control without σ1R primary antibody. Immunoprecipitated σ1Rs bound the IP₃R1 in control cells (b; cont) and in cells treated with Hμ or BDµ (b). In agreement, immunoprecipitated IP₃R1 bound σ1Rs in Hμ- and BDµ-treated cells (b). Western blot analysis documented amount of the σ1R protein in control cells, Hμ treated cells and BD 1047 (BDµ; 10 µmol/L) treated cells (c)