Fig. 5.

Concentration-dependence of haloperidol, BD 1047, Xestospongin C (Xest) and PRE-084 treatment on the number of neurites per cell (a, c), and neurite outgrowth (b, d–f). Experiments were performed on NG-108 cells differentiated by dbcAMP. Number of neurites in haloperidol-treated cells for 24 h decreased gradually from 0 to 100 µmol/L (a), while length of neurites increased (b). Length of neurites was lower in cells treated in parallel with haloperidol and Xest (1 µmol/L) (b; dashed). Similar results were observed with specific σ1R antagonist BD 1047 (c, d), which was used in the same concentrations as haloperidol. However, the effect of Xest (1 µmol/L) on BD 1047-treated cells varied according to the BD 1047 concentration (d). The σ1R agonist PRE-084 (e) and IP₃R blocker Xest (f) did not change the length of neurites in a concentration-dependent manner