Table 2.

Peptide-lipid monolayer interaction parameters. The data from Figure 1A were fitted using Equation 1, yielding the maximum surface pressure change, ΔΠ_max, and the dissociation constant, K_d, values presented here. The data on variation of the surface pressure of POPC:Chol 2:1 monolayer as a function of time after injection of VG peptides at a final concentration of 0.2 µM (Figure 1C) were fitted with Equation 2, permitting the calculation of the kinetic adsorption rate constant, k. Values are means ± standard error of the mean (SEM) of at least 3 experiments.

	VG-Chol	VG-PEG4-Chol	VG-PEG24-Chol
ΔΠmax (mN/m)	1.26 ± 0.38	4.16 ± 0.16	5.01 ± 0.80
Kd (10−2 µM)	9.83 ± 1.59	10.90 ± 1.59	30.89 ± 14.52
k (10−4 s−1)	2.06 ± 0.01	4.96 ± 0.05	2417 ± 767 1	7.34 ± 0.09 2

¹ The fit range was the first 20 s; ² the fit ranged from 20–2000 s