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Expression of PKG and CaV channel subunits in RIN-m5F cells. A) RT-PCR analysis of PKG cDNA expression in RIN-m5F cells. Total RNA from mouse whole brain was used as a positive control. Actin was used as an internal control. Primer sequences are indicated in Supplemental Table S1. B) Levels of different CaV channel subunit mRNAs expressed in RIN-m5F cells estimated from gene quantitative RT-PCR data and expressed as a number of copies of β-actin mRNA expression.
