Fig. 6.
Phosphorylation at sites S763 and S860 of CaV 1.3α1 subunit influences channel functional expression. A) Representative traces of Ba2+ currents recorded from HEK-293 cells expressing DPM mutant channels in absence and presence of 8-Br-cGMP (1 mM) plus 10 nM Calyculin A. B) Average current density-voltage relationships for IBa from HEK-293 cells expressing the wild-type CaV 1.3 channel and its double phosphorylation mutant (DPM) variant in the control condition and after incubation with 1 mM 8-Br-cGMP plus 10 nM Calyculin A (C). IBa density was calculated at a series of test pulses applied from a Vh of −80 mV in 5 mV steps between −70 and 30 mV.