Table 2.
Induction of drug oxidation activities of cultured BeWo cells under the recommended conditions.
| drug hydroxylation |
untreated | treated with | ||
|---|---|---|---|---|
|
| ||||
| phenobarbital | rifampicin | thalidomide | ||
|
| ||||
| pmol product formed/h/106 cells | ||||
| bupropion | 2.6 ± 0.4 | 3.4 ± 0.7 | 3.2 ± 0.8 | 2.7 ± 0.5 |
| midazolam (1´) | 60 ± 23 | 52 ± 31 | 120 ± 21* | 47 ± 11 |
| midazolam (4) | 1.3 ± 0.3 | 1.7 ± 0.3 | 1.3 ± 0.3 | 1.9 ± 0.4 |
| thalidomide (5) | 0.43 ± 0.17 | 0.62 ± 0.25 | 0.63 ± 0.25 | 0.51 ± 0.37 |
Data are means and SD values of triplicate determinations. BeWo cells were cultured in the recommended media containing 10% fetal bovine serum (v/v) with 20 µM rifampicin, 2000 µM phenobarbital, or 1000 µM thalidomide for 72 h. The medium was replaced with a fresh medium containing 50 µM midazolam, 100 µM bupropion, or 500 µM thalidomide and further incubated for 3 h.
*
p < 0.05, one way ANOVA with Bonferroni post tests compared to untreated group.