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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Prog Retin Eye Res. 2017 Nov 2;62:58–76. doi: 10.1016/j.preteyeres.2017.10.001

Fig. 19.

Fig. 19

Cryosections (A–D) and a whole mount retina (E–F) from 7 WG eyes. (A) Near the nerve head (NH), fusiform-shaped cells in the nerve fiber layer (NFL) were positive for both CXCR4 (green) and CD39 (red) (arrow in A–B). Inset shows the double-labeled cell indicated by the arrow in “A”–“B” at higher magnification. (DAPI counterstain showing the blue nuclei, red CD39 and green CXCR4 channels) (B) Same section without the DAPI channel shows CXCR4 immunostained cells the entire inner neuroblastic layer and some double-labeled cells in the nerve fiber layer express CXCR4. A few double-labeled cells are near the inner portion of the INL. (C) Same area in a serial section labeled for CXCR4 (green) and Ki67 (red). Only the outer neuroblastic layer is Ki67+. (D) Same section with the CXCR4 green channel turned off. (E) Retinal whole mount shows fusiform-shaped cells (arrows) near the optic nerve head (to the right and out of the field) that express both CD39 (red) and CXCR4 (green). (F) Same region as “E” with only the green CXCR4 channel shown. (A–D scale bar = 20 μm; E–F scale bar = 40 μm).