Figure 2.

Cytokine costimulated adaptive natural killer (NK) cells proficiently alert bystander cells in vitro. (A) Schematic illustration of experimental design. (B) Representative geoMFI of HLA class I on human umbilical vein endothelial cells (HUVEC) after 40 h treatment with indicated conditioned medium in the presence or absence of anti-interferon (IFN)-γ and anti-tumor necrosis factor (TNF) blocking antibodies (left) and summary of n = 8 treatments with conditioned medium from independent NK-cell stimulations (right). (C) Representative frequency of E-selectin⁺ HUVEC after 6 h treatment with indicated conditioned medium in the presence or absence of anti-TNF blocking antibodies (left) and summary of n = 8 treatments with conditioned medium from independent NK-cell stimulations (right). (D) Representative PSMB9 transcript abundance relative to GAPDH in HUVEC after 24 h treatment with indicated conditioned medium (left) and summary of n = 8 NK-cell stimulations (right). (E) Representative protein kinase R (PRK) PKR transcript abundance relative to GAPDH in HUVEC after 24 h treatment with indicated conditioned medium (left) and summary of n = 8 treatments with conditioned medium from independent NK-cell stimulations (right). Connected symbols indicate individual donors used to generate conditioned medium. All statistical analyses performed with one-tailed Wilcoxon matched-pairs test. NS, not significant; *p < 0.05 and **p < 0.01.