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. 2018 Jan 17;9:9. doi: 10.3389/fmicb.2018.00009

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Copyright © 2018 Hu, Zhou, Wang, Sun, Yarden and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ERG11 inhibition overrides the effects of erg2 deletion. After grown in liquid media for 13.5 h, the N. crassa wild-type and Δerg2 strains were treated with 2 mg/L ketoconazole for 22 h and same amount DMSO was used as a control. Transcript levels of cdr4 (NCU05591, encoding azole efflux pump CDR4), erg5 (NCU05278, encoding C-22 sterol desaturase), erg6 (NCU03006, encoding sterol C-24 methyl transferase), erg11 (NCU02624, encoding sterol 14α-demethylase) and erg24 (NCU08762, encoding C-14 sterol reductase) were measured by quantitative real-time polymerase chain reaction (qRT-PCR), and the expression was calculated by 2^-ΔΔCt method and normalized to β-tubulin. The results presented here are means of three biological replicates. The significant levels were calculated by t-test and marked as ^∗(p < 0.05), ^∗∗(p < 0.01), or ^∗∗∗(p < 0.001).