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. 2018 Jan 17;9:9. doi: 10.3389/fmicb.2018.00009

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Copyright © 2018 Hu, Zhou, Wang, Sun, Yarden and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sterol content between the N. crassa wild-type and Δcdr4 strain. After grown in liquid media for 13.5 h, the N. crassa wild-type and Δcdr4 strains were treated with 2 mg/L ketoconazole for 22 h and same amount DMSO was used as a control. KTC (A) and Sterols (B) were then extracted and measured by HPLC-MS. The abundance of sterols was calculated on the basis of the chromatogram peak area and normalized using an internal control and sample weight. The results presented here are means of three biological replicates. The significant levels were calculated by t-test and marked as ^∗(p < 0.05), ^∗∗(p < 0.01), or ^∗∗∗(p < 0.001).