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. 2018 Jan 17;7:542. doi: 10.3389/fcimb.2017.00542

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Copyright © 2018 Kumar, Singh, Abdin, Patel and Medigeshi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Capsid coimmunoprecipitates with DDX3X from cell lysates. (A) In-solution interaction of purified His-tagged DENV capsid and Huh-7 lysate was followed by pull-down of interacting proteins using Ni-NTA beads. Pull down with Huh-7 lysate alone is used as a control. (B) His-tagged DENV capsid and HA-DDX3X were co-transfected in HEK293T cells. 24 h p.t. lysate was prepared and capsid was immunoprecipitated using DENV capsid antibody. Total lysate and immunoprecipitated proteins were analyzed by western blots probed for capsid, HA and GAPDH. (C,D) Huh-7 cells were infected with DENV at an MOI of 5. At 24 h pi mock and DENV-infected Huh-7 cell lysates were prepared, endogenous DDX3X (C) or DENV capsid (D) was immunoprecipitated from total lysates using DDX3X or DENV capsid antibody respectively. Total lysate and immunoprecipitated proteins were analyzed by western blots probed for capsid and DDX3X.