Table 1.

Recipe for 3 mL of EdU staining solution.

Reagent	Volume
1X- PBS (pH 7.4)	2,241 μL
0.5 M L-Ascorbic acid (dissolved in Milli-Q water)	600 μL
2M Tris buffer (pH 8.5)	150 μL
100 mM Alexa Fluor Azide (dissolved in DMSO)	6 μL
1M Copper Sulfate (CuSO4)	3 μL
Total volume	3 mL