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Example parameters for OPT scanning of EdU and GFP in the Tg(mpeg1:gfp) transgenic line of a 6-month adult zebrafish brain.
| Visualization | Staining procedure | OPT laser | Exposure (ms) | Averaging | Rotation | Scan time |
|---|---|---|---|---|---|---|
| EdU | Alexa Fluor 647 Azide | 647 | ~200 | 2 | 0.45° | ~1-h |
| GFP | 1° Rabbit-anti-GFP (1:500) | 555 | ~150 | 2 | 0.45° | ~1-h |
| 2° Goat-anti-rabbit-Alexa555 (1:750) | ||||||
| Brain volume | none | 488 | ~800 | None | 0.45° | ~40-min |
