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. 2018 Jan 17;8:1132. doi: 10.3389/fphys.2017.01132

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Copyright © 2018 Fu, Li, Sun, Xun, Gao and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scatter diagram of leukocyte measured by flow cytometry in the peripheral blood of rat. Whole fresh blood samples anti-coagulated by EDTA.2Na were incubated with PE-conjugated anti-CD34 antibody and FITC-conjugated anti-vWF antibody. Then hemolysin was dissolved in distilled water, and applied to cells at the final concentration 10% (v/v). (A) The forward-scatter (FSC) and side-scatter (SSC) diagram, Cells in gate represent the mononuclear cells; (B) The fluorescence intensity scatter diagram. MNCs that were positively identified as CEPC by immunofluorescent staining for the PE⁺/FITC⁺ or CD34⁺/vWF⁺ (P2-Q2) represent EPC.