Figure 5.

Effect of TAPE on the expression of VEGF, eNOS, and MMP-9 in the myocardium of each group rats in 7 days after AMI. As mentioned above, rats in each group were randomly subdivided into seven subgroups, the 1st−7th subgroup. On the 1st−7th day next AMI modeling, rats in the 1st−7th subgroups were selected to be executed, respectively. Hearts were removed after animals were phlebotomized from their celiac artery under anesthesia. The LV tissue sample was taken at 2 mm under ligature, frozen in liquid nitrogen, and stored at −80°C before use. All myocardial protein samples were detected at the same time after all of rats had been executed and phlebotomized. The expression of VEGF, eNOS, and MMP-9 was analyzed by Western Blot. (A1,B1,C1), Western blot analysis of protein expression of VEGF, eNOS and MMP-9 in the myocardial tissue of each group rats; GAPDH was an internal reference protein for Western blot. (A2,B2,C2), Quantitative Western blot analysis of VEGF, eNOS and MMP-9 in myocardial tissue of each group rats; Ctrl, Isch, and Trich, the control group, the model group and the trich group, respectively; 1 d−7 d, represents the 1st−7th day next the coronary ligation or sham operation; ■, ■■, P < 0.05 or P < 0.01, compared with the Ctrl group; ^*, ^**, P < 0.05 or P < 0.01, compared with the Isch group. Δ, ΔΔ, P < 0.05 or P < 0.01, compared with the previous subgroup in the same group. Each data represents the average of group of 8 rats, and vertical lines indicate the standard error of the mean (SEM).