Figure 6.

Effect of TAPE on the plasma level of VEGF, eNOS, NO, and MMP-9 of each group rats in 7 days after AMI. Rats in each group were randomly subdivided into seven subgroups, the 1st−7th subgroup. On the 1st−7th day next AMI modeling, rats in the 1st−7th subgroups were selected to be phlebotomized from their celiac artery after being anesthetized by chloral hydrate, respectively. Whole fresh blood sample was centrifugalized 10 min at the speed of 3,000 r/min. The supernatant was stored at 4°C. All samples were tested at the same time after all of rats had been executed and phlebotomized. The plasma level of VEGF, eNOS, NO, and MMP-9 was detected by ELISA. Protocols reference to the manufacturer instruction of these ELISA kits. (A) the plasma content of VEGF in each group rats; (B) the plasma activity of eNOS in each group rats; (C) the plasma concentration of NO in each group rats; (D) the plasma activity of MMP-9 in each group rats. Ctrl, Isch, and Trich, the control group, the model group and the trich group respectively; 1 d−7 d, represents the 1st−7th day next the coronary ligation or sham operation; ■, ■■, P < 0.05 or P < 0.01, compared with the Ctrl group; ^*,^**, P < 0.05 or P < 0.01, compared with the Isch group. Δ, ΔΔ, P < 0.05 or P < 0.01, compared with the previous subgroup. Each data represents the average of group of 8 rats, and vertical lines indicate the standard error of the mean (SEM).