Expression of TNF-α, IL-6, IL-10 and IRAK-M in CLP+LPS and control mice. The concentration of (A) TNF-α, (B) IL-6 and (C) IL-10 in the supernatant of the culture medium of PBMCs was analyzed using ELISAs, and (D) IRAK-M expression was analyzed using reverse transcription-quantitative polymerase chain reaction in PBMCs from CLP-LPS mice (n=6) or control mice (n=6). The results are presented as the mean ± standard deviation. *P<0.05 as indicated. CLP, cecal ligation puncture procedure; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α; IL, interleukin; IRAK-M, interleukin-1 receptor-associated kinase 3; CLP-LPS, mice that underwent CLP surgery followed by LPS challenge; con, mice that underwent sham surgery followed by LPS challenge.