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. 2018 Jan 21;24(3):387–396. doi: 10.3748/wjg.v24.i3.387

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©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Representative strips after color development by line-blot immunoassay. The line-blot immunoassay strips had been coated with nine autoimmune liver disease-related antigens, including Ro-52, SLA/LP, LC-1, LKM-1, gp210, PML, Sp100, BPO and AMA-M2 (from left to right). BA group: ALD/93-57 with anti-BPO +; ALD/87-31 with anti-Ro-52 +++, anti-PML +, anti-Sp100 +, anti-BPO ++, and AMA-M2 +; ALD/89-53 with anti-BPO +; Other liver diseases group: Only ALD/198-59 with anti-LC-1 ±; Healthy group: All autoantibodies were negative. Positive control (ALD/52-75) showed anti-BPO +++ and AMA-M2 +++. Other liver diseases include choledochal cysts, transient cholestasis of unknown origin, and neonatal intrahepatic cholestasis caused by citrin deficiency. ALD: Autoimmune liver disease; BA: Biliary atresia; LC-1: Liver cytosolic antigen type 1.