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Increase of IFNγ, IL-17 and IL-4 secretion by splenocytes isolated from BALB/c mice immunized with vaccine. Splenocytes were cultured in vitro with HM. The supernatant of each group was harvested after 72 hr of culture and released IFNγ, IL-17, and IL-4 were measured by ELISA. These experiments were run in triplicate
