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. 2017 Oct 13;12(2):438–450. doi: 10.1038/ismej.2017.176

Figure 1.

Figure 1

D-arginine is released to the extracellular medium. (a) HPLC profile of Marfey’s derivatized preconditioned medium (PCM) from stationary-phase V. cholerae cultures for quantification of chiral amino acids. L- and D-forms were identified by comparison to standards. (b) Total D-amino acid content in wild-type and ΔbsrV mutant V. cholerae cultures, determined by D-amino acid oxidase assay. P-value <0.0001 (***). (c, d) HPLC analysis of Marfey’s derivatized PCM from wild-type (c) or ΔbsrV mutant (d) V. cholerae stationary-phase cultures. (e) Cell wall analysis of stationary-phase E. coli cultures grown in LB or the PCM from V. cholerae (Vc-PCM). Released D-Arg in Vc-PCM is incorporated into E.coli’s peptidoglycan pentapeptides (M5R). (f) Accumulation of M5R in wild-type V. cholerae, ΔbsrV mutant, E. coli and B. subtilis grown in LB and supplemented with 5 mm L-Arg or D-Arg. P-value <0.0001 (***).