Table I.

PCR conditions, primers and restrict enzyme used in the present study.

Gene	rs#	PCR condition			Primer sequence (5′-3′)	R/E
VEGF −2578C>A	rs699947	94°C	5 min	35 cycles	F: GGATGGGGCTGACTAGGTAAG	AvaII
		94°C	30 sec		R: AGCCCCCTTTTCCTCCAAC
		−62°C	30 sec
		−72°C	30 sec
		72°C	7 min
VEGF −1154G>A	rs1570360	94°C	5 min	38 cycles	F: CGCGTGTCTCTGGACAGAGTTTCC	MnlI
		94°C	30 sec		R: CGGGGACAGGCGAGCTTCAG
		−59°C	40 sec
		−72°C	30 sec
		72°C	5 min
VEGF −634C>G	rs2010963	94°C	5 min	40 cycles	F: CAGGTCACTCACTTTGCCCCGGTC	AvaII
		94°C	30 sec		R: GCTTGCCATTCCCCACTTGAATCG
		−63°C	35 sec
		−72°C	30 sec
		72°C	7 min
VEGF 936C>T	rs3025039	94°C	5 min	35 cycles	F: AAGGAAGAGGAGACTCTGCGCAGAGC	NlaIII
		94°C	30 sec		R: TAAATGTATGTATGTGGGTGGGTGTGTCTACAGG
		−68°C	1 min
		−72°C	30 sec
		72°C	7 min

PCR, polymerase chain reaction; rs#, RefSNP(rs) number; R/E, restriction enzyme; VEGF, vascular endothelial growth factor; F, forward primer; R, reverse primer.