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. 2017 Dec 15;15(2):1831–1838. doi: 10.3892/etm.2017.5642

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Copyright: © Ji et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — TAp73 modulated cyclin D1 regulation. (A) mRNA level of cyclin D1 in SGC7901, SNU-1 and SNU-5 parental and resistant cells was analyzed by reverse transcription-quantitative polymerase chain reaction. (B) The expression levels of the indicated proteins in SGC7901 parental and resistant cells were analyzed by western blot analysis. (C) Expression levels of the indicated proteins were analyzed by western blot analysis, and (D) apoptosis was evaluated by Hoechst 33258 staining in SGC7901 resistant cells transfected with TAp73 siRNA, followed by 20 nM Dox treatment. Error bars represent the standard deviation of triplicate detection. *P<0.05. Dox, doxorubicin; siRNA, small interfering RNA; Con, control.