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Effect of 20(S)-PPD on HepG2 cell apoptosis. HepG2 cells (4×105 per ml) were treated using (A) 0, (B) 40, (C) 50 and (D) 60 µM 20(S)-PPD for 24 h and were then stained using Annexin V-FITC and PI for flow cytometry analysis. Apoptotic cells were on the top right panels of each image. 20(S)-PPD, 20(S)-Protopanaxadiol; FITC, fluorescein isothiocyanate; PI, propidium iodide.
