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. 2017 Dec 5;15(2):1277–1284. doi: 10.3892/etm.2017.5594

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Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effect of 20(S)-PPD on apoptosis-associated proteins in HepG2 cells. (A) HepG2 cells (4×10⁵ per ml) were treated using 0, 40, 50 and 60 µM of 20(S)-PPD for 24 h and the expression of cleaved caspase-3, cleaved caspase-9 and PARP were determined using western blot analysis. Quantification of western blot analysis for (B) cleaved caspase-3, (C) cleaved caspase-9 and (D) PARP (top, 116 kDa) and cleaved PARP (bottom, 89 kDa). All values are expressed as the mean ± standard deviation. *P<0.05 vs. the control group. C, control group; 20(S)-PPD, 20(S)-Protopanaxadiol; PARP, poly (ADP-ribose) polymerase.